GP_BOT: plataforma hardware para la enseñanza de robótica en la titulación de ingeniería informática.

Versión electrónica de la ponencia presentada en V Congreso de Tecnologías Aplicadas a la Enseñanza de la Electrónica, celebrado en Las Palmas de Gran Canaria en 2002En este artículo se resumen las principales características de unas tarjetas basadas en el microcontrolador MC68HC908GP32 de Motorola, que se utilizan en el Laboratorio de la asignatura Robótica Autónoma en la carrera de Ingeniería Informática de la Universidad Autónoma de Madrid

Control de acceso mediante tarjeta chip: Aplicaciones de Control a través de Internet

Versión electrónica de la ponencia presentada en V Congreso de Tecnologías Aplicadas a la Enseñanza de la Electrónica, celebrado en Gran Canaria en 2002En este artículo se presenta un sistema real de control de accesos desarrollado por alumnos de nuestra Escuela. Este proyecto ha servido de plataforma para desarrollar los conocimientos no sólo de sistemas embebidos basados en microcontrolador, sino también para practicar las comunicaciones TCP/IP y el desarrollo de la base de datos de usuarios y su software de gestión

CellTag Indexing: Genetic barcode-based sample multiplexing for single-cell genomics

High-throughput single-cell assays increasingly require special consideration in experimental design, sample multiplexing, batch effect removal, and data interpretation. Here, we describe a lentiviral barcode-based multiplexing approach, CellTag Indexing, which uses predefined genetic barcodes that are heritable, enabling cell populations to be tagged, pooled, and tracked over time in the same experimental replicate. We demonstrate the utility of CellTag Indexing by sequencing transcriptomes using a variety of cell types, including long-term tracking of cell engraftment and differentiation in vivo. Together, this presents CellTag Indexing as a broadly applicable genetic multiplexing tool that is complementary with existing single-cell technologies

Retinoic acid and androgen receptors combine to achieve tissue specific control of human prostatic transglutaminase expression: a novel regulatory network with broader significance

In the human prostate, expression of prostate-specific genes is known to be directly regulated by the androgen–induced stimulation of the androgen receptor (AR). However, less is known about the expression control of the prostate-restricted TGM4 (hTGP) gene. In the present study we demonstrate that the regulation of the hTGP gene depends mainly on retinoic acid (RA). We provide evidence that the retinoic acid receptor gamma (RAR-G) plays a major role in the regulation of the hTGP gene and that presence of the AR, but not its transcriptional transactivation activity, is critical for hTGP transcription. RA and androgen responsive elements (RARE and ARE) were mapped to the hTGP promoter by chromatin immunoprecipitation (ChIP), which also indicated that the active ARE and RARE sites were adjacent, suggesting that the antagonistic effect of androgen and RA is related to the relative position of binding sites. Publicly available AR and RAR ChIP-seq data was used to find gene potentially regulated by AR and RAR. Four of these genes (CDCA7L, CDK6, BTG1 and SAMD3) were tested for RAR and AR binding and two of them (CDCA7L and CDK6) proved to be antagonistically regulated by androgens and RA confirming that this regulation is not particular of hTGP

Influence of the pairs in the expression of bullying in high school students in Arequipa

El objetivo del presente estudio fue determinar el grado de influencia que ejerce la cercanía de los alumnos de secundaria con pares que transgreden normas, sobre el rol que asumen estos alumnos dentro del bullying (agresor, víctima o espectador) en el área metropolitana de la ciudad de Arequipa, teniendo en cuenta sexo y grado de instrucción. Se evaluó a 3979 estudiantes de educación secundaria de colegios estatales, privados y paraestatales de 19 distritos de la provincia de Arequipa, con  edades comprendidas entre 11 y 19 años. Se realizó un análisis descriptivo y de regresión lineal múltiple y logística binaria para modelar la asociación entre variables. Los resultados muestran una mayor tendencia de los varones hacia las conductas agresivas y a asumir roles de “bully”. La influencia de pares transgresores de normas favorece en mayor grado la práctica de roles de agresor o víctima. En cuanto a la frecuencia de bullying, existe una alta prevalencia de agresores y víctimas entre los encuestados, cercana a la mitad de la muestra, mientras que la otra parte corresponde a los espectadores ya sean estos reforzadores del bullying o prosociales.The aim of this study was to determine the degree of influence of the proximity of high school students with peers who break rules on the role assumed these students within the bullying (bully, victim or viewer) in the metropolitan area of the city Arequipa, considering sex and level of education. We evaluated 3979 high school students from state, private and parastatal schools in 19 districts in the province of Arequipa, aged between 11 and 19 years. A descriptive and binary logistic regression and multiple linear analysis was performed to model the association between variables. The results show a greater tendency of men to aggressive behavior and assume roles of "bully". The influence of offenders pairs of rules favors greater practical role of aggressor or victim. As for the frequency of bullying, there is a high prevalence of perpetrators and victims among respondents, nearly half of the sample, while the other part is for the spectators whether these enhancers bullying or prosocial

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https://www.ester.ee/record=b4538881*es

Modelado computacional de datos epidemiológicos para predecir enfermedades de cultivos con base meteorológica

Para predecir el comportamiento de enfermedades de las plantas, mediante la construcción de modelos predictivos, se evaluó la severidad de manchas foliares, ocasionada por el hongo Altenaria tenuissima, en plantaciones de arándano alto (cultivar O’Neal) en tres localidades: San Pedro (S 33º 43' - W 059º 41'), Concordia (S 31º 24' - W 058º 02') y Gualeguaychú (S 33º 01' - W 058º 31'), durante los ciclos epidémicos primavero-estivo-otoñales de 2008/09 y 2009/10. Los mejores modelos simples de regresión logística de respuesta binaria integraron a Snc y a DTxnP (días con temperaturas entre 16 y 36°C), con precisiones de predicción de 93,8% y 78,5% respectivamente. El mejor modelo de respuesta ordinal integró a la interacción FPr*DTxnP (días con precipitación*días con temperaturas entre 16 y 36°C) y a Snc, con una precisión de predicción de 86,2%. La explicación biológica que subyace a los resultados obtenidos, refiere a un lento progreso epidémico primaveral, observado y simulado, contrastando con las altas tasas de incremento epidémico estivales, concordantes con hojas de senescencia creciente y alta frecuencia de días con registros térmicos entre 16 y 36ºC. Un adecuado ajuste y validación de los modelos predictivos obtenidos en esta investigación, permitirá el desarrollo de verdaderos sistemas de pronóstico, para la correcta gestión de la enfermedad, tanto desde el punto de vista técnico, como económico y ambiental.Sociedad Argentina de Informática e Investigación Operativ

Fueling Cancer Immunotherapy With Common Gamma Chain Cytokines

Adoptive T cell transfer therapy (ACT) using tumor infiltrating lymphocytes or lymphocytes redirected with antigen receptors (CAR or TCR) has revolutionized the field of cancer immunotherapy. Although CAR T cell therapy mediates robust responses in patients with hematological malignancies, this approach has been less effective for treating patients with solid tumors. Additionally, toxicities post T cell infusion highlight the need for safer ACT protocols. Current protocols traditionally expand T lymphocytes isolated from patient tumors or from peripheral blood to large magnitudes in the presence of high dose IL-2 prior to infusion. Unfortunately, this expansion protocol differentiates T cells to a full effector or terminal phenotype in vitro, consequently reducing their long-term survival and antitumor effectiveness in vivo. Post-infusion, T cells face further obstacles limiting their persistence and function within the suppressive tumor microenvironment. Therapeutic manipulation of T cells with common γ chain cytokines, which are critical growth factors for T cells, may be the key to bypass such immunological hurdles. Herein, we discuss the primary functions of the common γ chain cytokines impacting T cell survival and memory and then elaborate on how these distinct cytokines have been used to augment T cell-based cancer immunotherapy

Range expansion and the origin of USA300 north american epidemic methicillin-resistant Staphylococcus aureus

The USA300 North American epidemic (USA300-NAE) clone of methicillin-resistant Staphylococcus aureus has caused a wave of severe skin and soft tissue infections in the United States since it emerged in the early 2000s, but its geographic origin is obscure. Here we use the population genomic signatures expected from the serial founder effects of a geographic range expansion to infer the origin of USA300-NAE and identify polymorphisms associated with its spread. Genome sequences from 357 isolates from 22 U.S. states and territories and seven other countries are compared. We observe two significant signatures of range expansion, including decreases in genetic diversity and increases in derived allele frequency with geographic distance from the Pennsylvania region. These signatures account for approximately half of the core nucleotide variation of this clone, occur genome wide, and are robust to heterogeneity in temporal sampling of isolates, human population density, and recombination detection methods. The potential for positive selection of a gyrA fluoroquinolone resistance allele and several intergenic regions, along with a 2.4 times higher recombination rate in a resistant subclade, is noted. These results are the first to show a pattern of genetic variation that is consistent with a range expansion of an epidemic bacterial clone, and they highlight a rarely considered but potentially common mechanism by which genetic drift may profoundly influence bacterial genetic variation. IMPORTANCE The process of geographic spread of an origin population by a series of smaller populations can result in distinctive patterns of genetic variation. We detect these patterns for the first time with an epidemic bacterial clone and use them to uncover the clone’s geographic origin and variants associated with its spread. We study the USA300 clone of methicillin-resistant Staphylococcus aureus, which was first noticed in the early 2000s and subsequently became the leading cause of skin and soft tissue infections in the United States. The eastern United States is the most likely origin of epidemic USA300. Relatively few variants, which include an antibiotic resistance mutation, have persisted during this clone’s spread. Our study suggests that an early chapter in the genetic history of this epidemic bacterial clone was greatly influenced by random subsampling of isolates during the clone’s geographic spread

Genome-wide analyses implicate 33 loci in heritable dog osteosarcoma, including regulatory variants near CDKN2A/B

Background: Canine osteosarcoma is clinically nearly identical to the human disease, but is common and highly heritable, making genetic dissection feasible. Results: Through genome-wide association analyses in three breeds (greyhounds, Rottweilers, and Irish wolfhounds), we identify 33 inherited risk loci explaining 55% to 85% of phenotype variance in each breed. The greyhound locus exhibiting the strongest association, located 150 kilobases upstream of the genes CDKN2A/B, is also the most rearranged locus in canine osteosarcoma tumors. The top germline candidate variant is found at a >90% frequency in Rottweilers and Irish wolfhounds, and alters an evolutionarily constrained element that we show has strong enhancer activity in human osteosarcoma cells. In all three breeds, osteosarcoma-associated loci and regions of reduced heterozygosity are enriched for genes in pathways connected to bone differentiation and growth. Several pathways, including one of genes regulated by miR124, are also enriched for somatic copy-number changes in tumors. Conclusions: Mapping a complex cancer in multiple dog breeds reveals a polygenic spectrum of germline risk factors pointing to specific pathways as drivers of disease